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Perturbed structural dynamics underlie inhibition and altered efflux of the multidrug resistance pump AcrB

E Reading#*, Z Ahdash*, C Fais, V Ricci, X Wang-Kan, E Grimsey, JW Stone, G Malloci, AM Lau, H Findlay, A Konijnenberg, PJ Booth, P Ruggerone, AV Vargiu, LJV Piddock, A Politis# - #Senior and *Co-first authors

Nature Communications, 2020, 11, 5565. DOI: 10.1038/s41467-020-19397-2

Peptide-Based Approach to Inhibition of the Multidrug Resistance Efflux Pump AcrB

JA Jesin, TA Stone, CJ Mitchell, E Reading, CM Deber

Biochemistry, 2020, 59 (41), 3973-3981. DOI: 10.1021/acs.biochem.0c00417

Capturing membrane protein ribosome nascent-chain complexes in a native-like environment for co-translational studies

GA Pellowe, HE Findlay, K Lee, TM Gemeinhardt, LR Blackholly, E Reading#, PJ Booth# - # Senior author

Biochemistry, 2020, 59 (30), 2764-2775. DOI: 10.1021/acs.biochem.0c00423

Assessing Membrane Protein Structural Dynamics within Lipid Nanodiscs

E Reading

Trends in Biochemical Sciences, 2019, 44 (11), 989-990. DOI: 10.1016/j.tibs.2019.08.003

Native mass spectrometry goes more native: Investigation of membrane protein complexes directly from SMALPs
N Hellwig, O Peetz, Z Ahdash, I Tascón, PJ Booth, V Mikusevic, M Diskowski, A Politis, Y Hellmich, I Hänelt, E Reading, N Morgner
Chemical communications, 2018, 54 (97), 13702-13705. DOI: 10.1039/c8cc06284f

Direct protein-lipid interactions shape the conformational landscape of secondary transporters
C Martens, M Shekhar, AJ Borysik, AM Lau, E Reading, E Tajkhorshid, PJ Booth, A Politis
Nature communications, 2018, 9 (1), 4151. DOI: 10.1038/s41467-018-06704-1

Structural mass spectrometry of membrane proteins within their native lipid environments
E Reading
Chemistry–A European Journal, 2018, 24 (51), 13391-13398. DOI: 10.1002/chem.201801556

Photoinitiated Polymerization-Induced Self-Assembly in the Presence of Surfactants Enables Membrane Protein Incorporation into Vesicles
S Varlas, LD Blackman, HE Findlay, E Reading, PJ Booth, MI Gibson, R K O’Reilly
Macromolecules, 2018, 51 (16), 6190-6201. DOI: 10.1021/acs.macromol.8b00994

Lipid binding attenuates channel closure of the outer membrane protein OmpF
I Liko, MT Degiacomi, S Lee, TD Newport, J Gault, E Reading, JTS Hopper, NG Housden, P White, M Colledge, A Sula, BA Wallace, C Kleanthous, PJ Stansfeld, H Bayley, JLP Benesch, TM Allison, CV Robinson
Proceedings of the National Academy of Sciences, 2018, 115 (26), 6691-6696. DOI: 10.1073/pnas.1721152115  

Interrogating membrane protein conformational dynamics within native lipid compositions
E Reading#, Z Hall, C Martens, T Haghighi, H Findlay, Z Ahdash, A Politis, PJ Booth - # Senior author
Angewandte Chemie International Edition, 2017, 56 (49), 15654-15657. DOI: 10.1002/anie.201709657

Mechanistic insight into the assembly of the HerA–NurA helicase–nuclease DNA end resection complex
Z Ahdash, AM Lau, RT Byrne, K Lammens, A Stüetzer, H Urlaub, PJ Booth, E Reading, K-P Hopfner, A Politis
Nucleic acids research, 2017, 45 (20), 12025-12038. DOI: 10.1093/nar/gkx890

Structure formation during translocon-unassisted co-translational membrane protein folding
NJ Harris*, E Reading*, K Ataka, L Grzegorzewski, K Charalambous, X Liu, R Schlesinger, J Heberle, PJ Booth - * Co-first authors
Scientific reports, 2017, 7 (1), 8021. DOI: 10.1038/s41598-017-08522-9

Elucidation of drug metabolite structural isomers using molecular modeling coupled with ion mobility mass spectrometry
E Reading#, J Munoz-Muriedas, AD Roberts, GJ Dear, CV Robinson, C Beaumont# - # Co-senior authors
Analytical chemistry, 2016, 88 (4), 2273-2280. DOI: 10.1021/acs.analchem.5b04068

Quantifying the stabilizing effects of protein–ligand interactions in the gas phase
TM Allison, E Reading, I Liko, AJ Baldwin, A Laganowsky, CV Robinson
Nature communications, 2015, 6, 8551. DOI: 10.1038/ncomms9551

The effect of detergent, temperature, and lipid on the oligomeric state of MscL constructs: insights from mass spectrometry
E Reading, TA Walton, I Liko, MT Marty, A Laganowsky, DC Rees, C V Robinson
Chemistry & Biology, 2015, 22 (5), 593-603. DOI: 10.1016/j.chembiol.2015.04.016

The role of the detergent micelle in preserving the structure of membrane proteins in the gas phase
E Reading*, I Liko*, TM Allison, JLP Benesch, A Laganowsky, CV Robinson - * Co-first authors
Angewandte Chemie International Edition, 2015, 54 (15), 4577-4581. DOI: 10.1002/anie.201411622

Structure of a designed protein cage that self-assembles into a highly porous cube
YT Lai, E Reading, GL Hura, KL Tsai, A Laganowsky, FJ Asturias, JA Tainer, CV Robinson, TO Yeates
Nature chemistry, 2014, 6 (12), 1065. DOI: 10.1038/nchem.2107

Membrane proteins bind lipids selectively to modulate their structure and function
A Laganowsky*, E Reading*, TM Allison, MB Ulmschneider, MT Degiacomi, AJ Baldwin, CV Robinson - * Co-first authors
Nature, 2014, 510 (7503), 172. DOI: 10.1038/nature13419

Mass spectrometry reveals synergistic effects of nucleotides, lipids, and drugs binding to a multidrug resistance efflux pump
J Marcoux, SC Wang, A Politis, E Reading, J Ma, PC Biggin, M Zhou, H Tao, Q Zhang, G Chang, N Morgner, CV Robinson
Proceedings of the National Academy of Sciences, 2013, 110 (24), 9704-9709. DOI: 10.1073/pnas.1303888110

Mass spectrometry of intact membrane protein complexes
A Laganowsky, E Reading, JTS Hopper, CV Robinson
Nature protocols, 2013, 8 (4), 639. DOI: 10.1038/nprot.2013.024

Silver and Palladium Complexes Containing Ditopic N-Heterocyclic Carbene–Thione Ligands
M Slivarichova, E Reading, MF Haddow, H Othman, GR Owen
Organometallics, 2012, 31 (18), 6595-6607. DOI: 10.1021/om300615e


Detection of membrane proteins

A Laganowsky, E Reading

2015, US Patent App. 14/653,475