PUBLICATIONS & PATENTS

Conformational restriction shapes the inhibition of a multidrug efflux adaptor protein
B Russell Lewis, M R Uddin, M Moniruzzaman, K M Kuo, A J Higgins, L M N Shah, F Sobott, J M Parks, D Hammerschmid, J C Gumbart, H I Zgurskaya, E Reading
Nature Communications, (2023) 14, 3900. DOI: 10.1038/s41467-023-39615-x

TMEM106B is a receptor mediating ACE2-independent SARS-CoV-2 cell entry
J Baggen, M Jacquemyn, L Persoons, E Vanstreels, V E Pye, A G Wrobel, V Calvaresi, S R Martin, C Roustan, N B Cronin, E Reading, H J Thibaut, T Vercruysse, P Maes, F De Smet, A Yee, T Nivitchanyong, M Roell, N Franco-Hernandez, H Rhinn, A A Mamchak, M Ah Young-Chapon, E Brown, P Cherepanov, and D Daelemans
Cell, (2023) 186, 1–16. DOI: https://doi.org/10.1016/j.cell.2023.06.005

Structural dynamics in the evolution of SARS-CoV-2 spike glycoprotein
V Calvaresi, A Wrobel, J Toporowska, D Hammerschmid, K Doores, R Bradshaw, R Parsons, D Benton, C Roustan, E Reading, M Malim, S Gamblin, A Politis
Nature Communications, (2023) 14, 1421. DOI: 10.1038/s41467-023-36745-0

Chromatographic phospholipid trapping for automated H/D exchange mass spectrometry of membrane protein-lipid assemblies
D Hammerschmid, V Calvaresi, C Bailey, B Russell Lewis, A Politis, M Morris, L Denbigh, M Anderson, E Reading
Analytical Chemistry, (2023) 95, 5, 3002–3011. DOI: 10.1021/acs.analchem.2c04876

Structural mass spectrometry approaches to understand multidrug efflux systems
B Russell Lewis, R Lawrence, D Hammerschmid, E Reading
Essays In Biochemistry, (2023) 67, 255–267. DOI: 10.1042/EBC20220190

Cell-Free Synthesis Strategies to Probe Co-translational Folding of Proteins Within Lipid Membranes
NJ Harris, E Reading, PJ Booth
Methods in Molecular Biology, (2022) 2433, 273-292. DOI: 10.1007/978-1-0716-1998-8_17

Perturbed structural dynamics underlie inhibition and altered efflux of the multidrug resistance pump AcrB
E Reading, Z Ahdash, C Fais, V Ricci, X Wang-Kan, E Grimsey, JW Stone, G Malloci, AM Lau, H Findlay, A Konijnenberg, PJ Booth, P Ruggerone, AV Vargiu, LJV Piddock, A Politis 
Nature Communications, (2020) 11, 5565. DOI: 10.1038/s41467-020-19397-2
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Peptide-Based Approach to Inhibition of the Multidrug Resistance Efflux Pump AcrB
JA Jesin, TA Stone, CJ Mitchell, E Reading, CM Deber
Biochemistry, (2020) 59, 41, 3973-3981. DOI: 10.1021/acs.biochem.0c00417
 
Capturing membrane protein ribosome nascent-chain complexes in a native-like environment for co-translational studies
GA Pellowe, HE Findlay, K Lee, TM Gemeinhardt, LR Blackholly, E Reading, PJ Booth
Biochemistry, (2020) 59, 30, 2764-2775. DOI: 10.1021/acs.biochem.0c00423
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Assessing Membrane Protein Structural Dynamics within Lipid Nanodiscs
E Reading
Trends in Biochemical Sciences, (2019) 44, 11, 989-990. DOI: 10.1016/j.tibs.2019.08.003
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Native mass spectrometry goes more native: Investigation of membrane protein complexes directly from SMALPs
N Hellwig, O Peetz, Z Ahdash, I Tascón, PJ Booth, V Mikusevic, M Diskowski, A Politis, Y Hellmich, I Hänelt, E Reading, N Morgner
Chemical communications, (2018) 54, 97, 13702-13705. DOI: 10.1039/c8cc06284f
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Direct protein-lipid interactions shape the conformational landscape of secondary transporters
C Martens, M Shekhar, AJ Borysik, AM Lau, E Reading, E Tajkhorshid, PJ Booth, A Politis
Nature communications, (2018) 9, 1, 4151. DOI: 10.1038/s41467-018-06704-1
 
Structural mass spectrometry of membrane proteins within their native lipid environments
E Reading
Chemistry–A European Journal, (2018) 24, 51, 13391-13398. DOI: 10.1002/chem.201801556
 
Photoinitiated Polymerization-Induced Self-Assembly in the Presence of Surfactants Enables Membrane Protein Incorporation into Vesicles
S Varlas, LD Blackman, HE Findlay, E Reading, PJ Booth, MI Gibson, R K O’Reilly
Macromolecules, (2018) 51, 16, 6190-6201. DOI: 10.1021/acs.macromol.8b00994
 
Lipid binding attenuates channel closure of the outer membrane protein OmpF
I Liko, MT Degiacomi, S Lee, TD Newport, J Gault, E Reading, JTS Hopper, NG Housden, P White, M Colledge, A Sula, BA Wallace, C Kleanthous, PJ Stansfeld, H Bayley, JLP Benesch, TM Allison, CV Robinson
Proceedings of the National Academy of Sciences, (2018) 115, 26, 6691-6696. DOI: 10.1073/pnas.1721152115  
 
Interrogating membrane protein conformational dynamics within native lipid compositions
E Reading, Z Hall, C Martens, T Haghighi, H Findlay, Z Ahdash, A Politis, PJ Booth
Angewandte Chemie International Edition, (2017) 56, 49, 15654-15657. DOI: 10.1002/anie.201709657
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Mechanistic insight into the assembly of the HerA–NurA helicase–nuclease DNA end resection complex
Z Ahdash, AM Lau, RT Byrne, K Lammens, A Stüetzer, H Urlaub, PJ Booth, E Reading, K-P Hopfner, A Politis
Nucleic acids research, (2017) 45, 20, 12025-12038. DOI: 10.1093/nar/gkx890
 
Structure formation during translocon-unassisted co-translational membrane protein folding
NJ Harris, E Reading, K Ataka, L Grzegorzewski, K Charalambous, X Liu, R Schlesinger, J Heberle, PJ Booth
Scientific reports, (2017) 7, 1, 8021. DOI: 10.1038/s41598-017-08522-9
 
Elucidation of drug metabolite structural isomers using molecular modeling coupled with ion mobility mass spectrometry
E Reading, J Munoz-Muriedas, AD Roberts, GJ Dear, CV Robinson, C Beaumont
Analytical chemistry, (2016) 88, 4, 2273-2280. DOI: 10.1021/acs.analchem.5b04068
 
​Quantifying the stabilizing effects of protein–ligand interactions in the gas phase
TM Allison, E Reading, I Liko, AJ Baldwin, A Laganowsky, CV Robinson
Nature communications, (2015) 6, 8551. DOI: 10.1038/ncomms9551
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The effect of detergent, temperature, and lipid on the oligomeric state of MscL constructs: insights from mass spectrometry
E Reading, TA Walton, I Liko, MT Marty, A Laganowsky, DC Rees, C V Robinson
Chemistry & Biology, (2015) 22, 5, 593-603. DOI: 10.1016/j.chembiol.2015.04.016
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The role of the detergent micelle in preserving the structure of membrane proteins in the gas phase
E Reading, I Liko, TM Allison, JLP Benesch, A Laganowsky, CV Robinson
Angewandte Chemie International Edition, (2015) 54, 15, 4577-4581. DOI: 10.1002/anie.201411622
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Structure of a designed protein cage that self-assembles into a highly porous cube
YT Lai, E Reading, GL Hura, KL Tsai, A Laganowsky, FJ Asturias, JA Tainer, CV Robinson, TO Yeates
Nature chemistry, (2014), 6, 12, 1065. DOI: 10.1038/nchem.2107
 
Membrane proteins bind lipids selectively to modulate their structure and function
A Laganowsky, E Reading, TM Allison, MB Ulmschneider, MT Degiacomi, AJ Baldwin, CV Robinson
Nature, (2014) 510, 7503, 172. DOI: 10.1038/nature13419
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Mass spectrometry reveals synergistic effects of nucleotides, lipids, and drugs binding to a multidrug resistance efflux pump
J Marcoux, SC Wang, A Politis, E Reading, J Ma, PC Biggin, M Zhou, H Tao, Q Zhang, G Chang, N Morgner, CV Robinson
Proceedings of the National Academy of Sciences, (2013) 110, 24, 9704-9709. DOI: 10.1073/pnas.1303888110
 
Mass spectrometry of intact membrane protein complexes
A Laganowsky, E Reading, JTS Hopper, CV Robinson
Nature protocols, (2013) 8, 4, 639. DOI: 10.1038/nprot.2013.024
 
Silver and Palladium Complexes Containing Ditopic N-Heterocyclic Carbene–Thione Ligands
M Slivarichova, E Reading, MF Haddow, H Othman, GR Owen
Organometallics, (2012) 31, 18, 6595-6607. DOI: 10.1021/om300615e

Patents
Reading E, Hammerschmid D, Howarth M, Keeble AH.
(2023) UK Intellectual Property Office 2315107.9

Detection of membrane proteins
A Laganowsky, E Reading
(2015) US Patent App. 14/653,475