PUBLICATIONS & PATENTS

Mg2+-dependent mechanism of environmental versatility in a multidrug efflux pump
B Russell Lewis, MR Uddin, KM Kuo, LMN Shah, NJ Harris, PJ Booth, D Hammerschmid, JC Gumbart, HI Zgurskaya, E Reading
Structure (2024) Online, DOI: 10.1016/j.str.2024.12.012

Conformational restriction shapes the inhibition of a multidrug efflux adaptor protein
B Russell Lewis, MR Uddin, M Moniruzzaman, KM Kuo, AJ Higgins, LMN Shah, F Sobott, JM Parks, D Hammerschmid, JC Gumbart, HI Zgurskaya, E Reading
Nature Communications, (2023) 14, 3900. DOI: 10.1038/s41467-023-39615-x

TMEM106B is a receptor mediating ACE2-independent SARS-CoV-2 cell entry
J Baggen, M Jacquemyn, L Persoons, E Vanstreels, VE Pye, AG Wrobel, V Calvaresi, SR Martin, C Roustan, NB Cronin, E Reading, HJ Thibaut, T Vercruysse, P Maes, F De Smet, A Yee, T Nivitchanyong, M Roell, N Franco-Hernandez, H Rhinn, AA Mamchak, M Ah Young-Chapon, E Brown, P Cherepanov, and D Daelemans
Cell, (2023) 186, 1–16. DOI: https://doi.org/10.1016/j.cell.2023.06.005

Structural dynamics in the evolution of SARS-CoV-2 spike glycoprotein
V Calvaresi, A Wrobel, J Toporowska, D Hammerschmid, K Doores, R Bradshaw, R Parsons, D Benton, C Roustan, E Reading, M Malim, S Gamblin, A Politis
Nature Communications, (2023) 14, 1421. DOI: 10.1038/s41467-023-36745-0

Chromatographic phospholipid trapping for automated H/D exchange mass spectrometry of membrane protein-lipid assemblies
D Hammerschmid, V Calvaresi, C Bailey, B Russell Lewis, A Politis, M Morris, L Denbigh, M Anderson, E Reading
Analytical Chemistry, (2023) 95, 5, 3002–3011. DOI: 10.1021/acs.analchem.2c04876

Structural mass spectrometry approaches to understand multidrug efflux systems
B Russell Lewis, R Lawrence, D Hammerschmid, E Reading
Essays In Biochemistry, (2023) 67, 255–267. DOI: 10.1042/EBC20220190

Cell-Free Synthesis Strategies to Probe Co-translational Folding of Proteins Within Lipid Membranes
NJ Harris, E Reading, PJ Booth
Methods in Molecular Biology, (2022) 2433, 273-292. DOI: 10.1007/978-1-0716-1998-8_17

Perturbed structural dynamics underlie inhibition and altered efflux of the multidrug resistance pump AcrB
E Reading, Z Ahdash, C Fais, V Ricci, X Wang-Kan, E Grimsey, JW Stone, G Malloci, AM Lau, H Findlay, A Konijnenberg, PJ Booth, P Ruggerone, AV Vargiu, LJV Piddock, A Politis 
Nature Communications, (2020) 11, 5565. DOI: 10.1038/s41467-020-19397-2
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Peptide-Based Approach to Inhibition of the Multidrug Resistance Efflux Pump AcrB
JA Jesin, TA Stone, CJ Mitchell, E Reading, CM Deber
Biochemistry, (2020) 59, 41, 3973-3981. DOI: 10.1021/acs.biochem.0c00417
 
Capturing membrane protein ribosome nascent-chain complexes in a native-like environment for co-translational studies
GA Pellowe, HE Findlay, K Lee, TM Gemeinhardt, LR Blackholly, E Reading, PJ Booth
Biochemistry, (2020) 59, 30, 2764-2775. DOI: 10.1021/acs.biochem.0c00423
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Assessing Membrane Protein Structural Dynamics within Lipid Nanodiscs
E Reading
Trends in Biochemical Sciences, (2019) 44, 11, 989-990. DOI: 10.1016/j.tibs.2019.08.003
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Native mass spectrometry goes more native: Investigation of membrane protein complexes directly from SMALPs
N Hellwig, O Peetz, Z Ahdash, I Tascón, PJ Booth, V Mikusevic, M Diskowski, A Politis, Y Hellmich, I Hänelt, E Reading, N Morgner
Chemical communications, (2018) 54, 97, 13702-13705. DOI: 10.1039/c8cc06284f
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Direct protein-lipid interactions shape the conformational landscape of secondary transporters
C Martens, M Shekhar, AJ Borysik, AM Lau, E Reading, E Tajkhorshid, PJ Booth, A Politis
Nature communications, (2018) 9, 1, 4151. DOI: 10.1038/s41467-018-06704-1
 
Structural mass spectrometry of membrane proteins within their native lipid environments
E Reading
Chemistry–A European Journal, (2018) 24, 51, 13391-13398. DOI: 10.1002/chem.201801556
 
Photoinitiated Polymerization-Induced Self-Assembly in the Presence of Surfactants Enables Membrane Protein Incorporation into Vesicles
S Varlas, LD Blackman, HE Findlay, E Reading, PJ Booth, MI Gibson, R K O’Reilly
Macromolecules, (2018) 51, 16, 6190-6201. DOI: 10.1021/acs.macromol.8b00994
 
Lipid binding attenuates channel closure of the outer membrane protein OmpF
I Liko, MT Degiacomi, S Lee, TD Newport, J Gault, E Reading, JTS Hopper, NG Housden, P White, M Colledge, A Sula, BA Wallace, C Kleanthous, PJ Stansfeld, H Bayley, JLP Benesch, TM Allison, CV Robinson
Proceedings of the National Academy of Sciences, (2018) 115, 26, 6691-6696. DOI: 10.1073/pnas.1721152115  
 
Interrogating membrane protein conformational dynamics within native lipid compositions
E Reading, Z Hall, C Martens, T Haghighi, H Findlay, Z Ahdash, A Politis, PJ Booth
Angewandte Chemie International Edition, (2017) 56, 49, 15654-15657. DOI: 10.1002/anie.201709657
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Mechanistic insight into the assembly of the HerA–NurA helicase–nuclease DNA end resection complex
Z Ahdash, AM Lau, RT Byrne, K Lammens, A Stüetzer, H Urlaub, PJ Booth, E Reading, K-P Hopfner, A Politis
Nucleic acids research, (2017) 45, 20, 12025-12038. DOI: 10.1093/nar/gkx890
 
Structure formation during translocon-unassisted co-translational membrane protein folding
NJ Harris, E Reading, K Ataka, L Grzegorzewski, K Charalambous, X Liu, R Schlesinger, J Heberle, PJ Booth
Scientific reports, (2017) 7, 1, 8021. DOI: 10.1038/s41598-017-08522-9
 
Elucidation of drug metabolite structural isomers using molecular modeling coupled with ion mobility mass spectrometry
E Reading, J Munoz-Muriedas, AD Roberts, GJ Dear, CV Robinson, C Beaumont
Analytical chemistry, (2016) 88, 4, 2273-2280. DOI: 10.1021/acs.analchem.5b04068
 
​Quantifying the stabilizing effects of protein–ligand interactions in the gas phase
TM Allison, E Reading, I Liko, AJ Baldwin, A Laganowsky, CV Robinson
Nature communications, (2015) 6, 8551. DOI: 10.1038/ncomms9551
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The effect of detergent, temperature, and lipid on the oligomeric state of MscL constructs: insights from mass spectrometry
E Reading, TA Walton, I Liko, MT Marty, A Laganowsky, DC Rees, C V Robinson
Chemistry & Biology, (2015) 22, 5, 593-603. DOI: 10.1016/j.chembiol.2015.04.016
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The role of the detergent micelle in preserving the structure of membrane proteins in the gas phase
E Reading, I Liko, TM Allison, JLP Benesch, A Laganowsky, CV Robinson
Angewandte Chemie International Edition, (2015) 54, 15, 4577-4581. DOI: 10.1002/anie.201411622
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Structure of a designed protein cage that self-assembles into a highly porous cube
YT Lai, E Reading, GL Hura, KL Tsai, A Laganowsky, FJ Asturias, JA Tainer, CV Robinson, TO Yeates
Nature chemistry, (2014), 6, 12, 1065. DOI: 10.1038/nchem.2107
 
Membrane proteins bind lipids selectively to modulate their structure and function
A Laganowsky, E Reading, TM Allison, MB Ulmschneider, MT Degiacomi, AJ Baldwin, CV Robinson
Nature, (2014) 510, 7503, 172. DOI: 10.1038/nature13419
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Mass spectrometry reveals synergistic effects of nucleotides, lipids, and drugs binding to a multidrug resistance efflux pump
J Marcoux, SC Wang, A Politis, E Reading, J Ma, PC Biggin, M Zhou, H Tao, Q Zhang, G Chang, N Morgner, CV Robinson
Proceedings of the National Academy of Sciences, (2013) 110, 24, 9704-9709. DOI: 10.1073/pnas.1303888110
 
Mass spectrometry of intact membrane protein complexes
A Laganowsky, E Reading, JTS Hopper, CV Robinson
Nature protocols, (2013) 8, 4, 639. DOI: 10.1038/nprot.2013.024
 
Silver and Palladium Complexes Containing Ditopic N-Heterocyclic Carbene–Thione Ligands
M Slivarichova, E Reading, MF Haddow, H Othman, GR Owen
Organometallics, (2012) 31, 18, 6595-6607. DOI: 10.1021/om300615e

Patents
Polypeptides and peptide tags capable of spontaneous isopeptide bond formation at low temperature and low pH and uses thereof 
Reading E, Hammerschmid D, Howarth M, Keeble AH.
(2024) International (PCT) Patent Application No. PCT/GB2024/052526

Detection of membrane proteins
A Laganowsky, E Reading
(2015) US Patent App. 14/653,475